Journal: Oncoimmunology
Article Title: Spatial heterogeneity of T cell repertoire across NSCLC tumors, tumor edges, adjacent and distant lung tissues
doi: 10.1080/2162402X.2023.2233399
Figure Lengend Snippet: Immunohistochemistry on T cell markers in tumor, tumor edge, 1 cm, 2 cm, 5 cm, and 10 cm adjacent normal tissues of early NSCLC patients. a: the representative images of immunohistochemical staining on CD4, CD8, and PD-L1 in tumor, tumor edge, 1 cm, 2 cm, 5 cm, and 10 cm adjacent normal tissues. b: CD8+ cell density (cells/mm 2 ) measured by IHC across all tissues for n = 4 patients. c: CD4+ cell density (cells/mm 2 ) measured by IHC across all tissues for n = 4 patients. d: Boxplot represents the comparison of CD8+ cell density across all tissues. Mann-Whitney U-test was used for statistical analysis. e: Boxplot represents the comparison of CD4+ cell density across all tissues. Mann-Whitney U-test was used for statistical analysis. f: Median CD8+: CD4+ cell densities (cells/mm 2 ) across all regions for n = 4 patients. g: PD-L1 score measured by IHC across all tissues for n = 4 patients. h: Boxplot represents the comparison of PD-L1 score across all tissues. Mann-Whitney U-test was used for statistical analysis. i: the representative images of multiplex immunohistochemistry show the expression of CKpan, TIM3, PD1, LAG3, and CTLA4 in tumor, tumor edge, and 1 cm, 2 cm, 5 cm, and 10 cm adjacent normal tissues. Scale bar, 100 μm.
Article Snippet: Immunohistochemistry score of CD4 and CD8 positive staining were quantified by artificial intelligence-assisted IHC scoring system (Servicebio Inc, Wuhan, China).
Techniques: Immunohistochemistry, Staining, Comparison, MANN-WHITNEY, Multiplex Assay, Expressing